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puc19 addgene addgene  (Addgene inc)


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    Addgene inc puc19 addgene addgene
    Puc19 Addgene Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc19+addgene+addgene/pUC19+(Plasmid+%2350005)/pm40829587-803-51-52
    Average 96 stars, based on 224 article reviews
    puc19 addgene addgene - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    CRISPR:

    Article Title: The small GTPase Ran defines nuclear pore complex asymmetry.
    Article Snippet: GFP[E.3xP3]=vas-Cas9}VK00027 Bloomington Drosophila Stock center68 RRID: BDSC#51324FBtpi0154822 D.melanogaster: yw; nos-Cas9(II-attp-40 Bestgene Inc. Kondo et al. 201369 N/A D.melanogaster: y2; Nup214::emGFP; This study N/A D.melanogaster: w[1118]; mCherry::Nup358 This study N/A D.melanogaster: w[1118]; emGFP::Nup98 This study N/A D.melanogaster: w[1118], mCherry::Nup153;; This study N/A D.melanogaster: w[1118]; emGFP::Nup133 This study N/A D.melanogaster: y2; Nup358::emGFP This study N/A D.melanogaster: w[1118]; Nup358::VN/TM3,Sb,Ser This study N/A D.melanogaster: w[1118]; Nup358::VC This study N/A (Continued on next page) ll OPEN ACCESS e2 Cell 188, 5931–5946.e1–e9, October 16, 2025 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER S. cerevisiae; MATa, ura3Δ0, leu2Δ0, his3Δ1, met15Δ0, Δmot2::kanMX4, NUP188::EGFP::HIS3MX6, NUP170::mars::hphNT1 This study N/A Oligonucleotides Guide RNA targeting sequences for Drosophila CRISPR editing, see Table S1 This study N/A Recombinant DNA cDNA pOT-LD43045 Drosophila Genomics Resource Center DGRC#LD43045FlybaseID: FBcl0176185 pU6-Bbsl-chiRNA Addgene Addgene #45946 pHD-DsRed Addgene Addgene #51434 pUC19 Addgene Addgene #50005 pGeEX_6P1 Amersham AmershamCat# 27-4597-01 Software and algorithms Fiji Schindelin et al. 201370 http://fiji.sc Icy de Chaumont et al. 201271 http://icy.bioimageanalysis.org/ Ec-CLEM (Icy plugin) Paul-Gilloteaux et al. 201272 http://icy.bioimageanalysis.org/ plugin/ec-clem/ SerialEM Mastronade 200573 https://bio3d.colorado.edu/SerialEM/ SerialFIB Klumpe et al. 202174 https://github.com/sklumpe/SerialFIB gctf Zhang et al. 201675 N/A novaCTF Turo�nová et al. 201776 https://github.com/turonova/novaCTF novaSTA Turonova 202277 https://github.com/turonova/novaSTA GAPSTOPTM Turonova 202478 https://doi.org/10.5281/zenodo.10822455 Matlab The MathWorks, Inc. https://www.mathworks.com/ products/matlab.html Imod Kremer et al. 199679 https://bio3d.colorado.edu/imod/ AreTomo Zheng et al. 202280 https://msg.ucsf.edu/software MemBrain-Seg Lamm et al. 202281 https://github.com/teamtomo/ membrain-seg cryoCAT Turo�nová, B. https://github.com/ turonova/cryoCAT https://github.com/turonova/cryoCAT Relion 3.1 Zivanov et al. 201882 https://relion.readthedocs.io/ en/release-3.1/ ArtiaX Ermel et al. 202283 https://github.com/FrangakisLab/ArtiaX ChimeraX Meng et al. 202384 https://www.cgl.ucsf.edu/chimerax/ Python Python.org https://www.python.org/ SciPy Virtanen et al. 202085 https://scipy.org matplotlib Hunter et al. 200786 https://matplotlib.org/ napari Napari contributors 202598 https://napari.org/ Other Pelco easiGlow Ted Pella, Inc. N/A R1/4 SiO2 gold 200 mesh grids Quantifoil N/A Leica EM GP2 Leica Microsystems N/A Aquilos 1 cryo-FIB-SEM Thermo Scientific N/A Aquilos Leica CLEM Shuttle Thermo Scientific N/A Stellaris SP8 cryoCLEM Leica N/A (Continued on next page) ll OPEN ACCESS Cell 188, 5931–5946.e1–e9, October 16, 2025 e3 Article ..

    Recombinant:

    Article Title: The small GTPase Ran defines nuclear pore complex asymmetry.
    Article Snippet: GFP[E.3xP3]=vas-Cas9}VK00027 Bloomington Drosophila Stock center68 RRID: BDSC#51324FBtpi0154822 D.melanogaster: yw; nos-Cas9(II-attp-40 Bestgene Inc. Kondo et al. 201369 N/A D.melanogaster: y2; Nup214::emGFP; This study N/A D.melanogaster: w[1118]; mCherry::Nup358 This study N/A D.melanogaster: w[1118]; emGFP::Nup98 This study N/A D.melanogaster: w[1118], mCherry::Nup153;; This study N/A D.melanogaster: w[1118]; emGFP::Nup133 This study N/A D.melanogaster: y2; Nup358::emGFP This study N/A D.melanogaster: w[1118]; Nup358::VN/TM3,Sb,Ser This study N/A D.melanogaster: w[1118]; Nup358::VC This study N/A (Continued on next page) ll OPEN ACCESS e2 Cell 188, 5931–5946.e1–e9, October 16, 2025 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER S. cerevisiae; MATa, ura3Δ0, leu2Δ0, his3Δ1, met15Δ0, Δmot2::kanMX4, NUP188::EGFP::HIS3MX6, NUP170::mars::hphNT1 This study N/A Oligonucleotides Guide RNA targeting sequences for Drosophila CRISPR editing, see Table S1 This study N/A Recombinant DNA cDNA pOT-LD43045 Drosophila Genomics Resource Center DGRC#LD43045FlybaseID: FBcl0176185 pU6-Bbsl-chiRNA Addgene Addgene #45946 pHD-DsRed Addgene Addgene #51434 pUC19 Addgene Addgene #50005 pGeEX_6P1 Amersham AmershamCat# 27-4597-01 Software and algorithms Fiji Schindelin et al. 201370 http://fiji.sc Icy de Chaumont et al. 201271 http://icy.bioimageanalysis.org/ Ec-CLEM (Icy plugin) Paul-Gilloteaux et al. 201272 http://icy.bioimageanalysis.org/ plugin/ec-clem/ SerialEM Mastronade 200573 https://bio3d.colorado.edu/SerialEM/ SerialFIB Klumpe et al. 202174 https://github.com/sklumpe/SerialFIB gctf Zhang et al. 201675 N/A novaCTF Turo�nová et al. 201776 https://github.com/turonova/novaCTF novaSTA Turonova 202277 https://github.com/turonova/novaSTA GAPSTOPTM Turonova 202478 https://doi.org/10.5281/zenodo.10822455 Matlab The MathWorks, Inc. https://www.mathworks.com/ products/matlab.html Imod Kremer et al. 199679 https://bio3d.colorado.edu/imod/ AreTomo Zheng et al. 202280 https://msg.ucsf.edu/software MemBrain-Seg Lamm et al. 202281 https://github.com/teamtomo/ membrain-seg cryoCAT Turo�nová, B. https://github.com/ turonova/cryoCAT https://github.com/turonova/cryoCAT Relion 3.1 Zivanov et al. 201882 https://relion.readthedocs.io/ en/release-3.1/ ArtiaX Ermel et al. 202283 https://github.com/FrangakisLab/ArtiaX ChimeraX Meng et al. 202384 https://www.cgl.ucsf.edu/chimerax/ Python Python.org https://www.python.org/ SciPy Virtanen et al. 202085 https://scipy.org matplotlib Hunter et al. 200786 https://matplotlib.org/ napari Napari contributors 202598 https://napari.org/ Other Pelco easiGlow Ted Pella, Inc. N/A R1/4 SiO2 gold 200 mesh grids Quantifoil N/A Leica EM GP2 Leica Microsystems N/A Aquilos 1 cryo-FIB-SEM Thermo Scientific N/A Aquilos Leica CLEM Shuttle Thermo Scientific N/A Stellaris SP8 cryoCLEM Leica N/A (Continued on next page) ll OPEN ACCESS Cell 188, 5931–5946.e1–e9, October 16, 2025 e3 Article ..

    Software:

    Article Title: The small GTPase Ran defines nuclear pore complex asymmetry.
    Article Snippet: GFP[E.3xP3]=vas-Cas9}VK00027 Bloomington Drosophila Stock center68 RRID: BDSC#51324FBtpi0154822 D.melanogaster: yw; nos-Cas9(II-attp-40 Bestgene Inc. Kondo et al. 201369 N/A D.melanogaster: y2; Nup214::emGFP; This study N/A D.melanogaster: w[1118]; mCherry::Nup358 This study N/A D.melanogaster: w[1118]; emGFP::Nup98 This study N/A D.melanogaster: w[1118], mCherry::Nup153;; This study N/A D.melanogaster: w[1118]; emGFP::Nup133 This study N/A D.melanogaster: y2; Nup358::emGFP This study N/A D.melanogaster: w[1118]; Nup358::VN/TM3,Sb,Ser This study N/A D.melanogaster: w[1118]; Nup358::VC This study N/A (Continued on next page) ll OPEN ACCESS e2 Cell 188, 5931–5946.e1–e9, October 16, 2025 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER S. cerevisiae; MATa, ura3Δ0, leu2Δ0, his3Δ1, met15Δ0, Δmot2::kanMX4, NUP188::EGFP::HIS3MX6, NUP170::mars::hphNT1 This study N/A Oligonucleotides Guide RNA targeting sequences for Drosophila CRISPR editing, see Table S1 This study N/A Recombinant DNA cDNA pOT-LD43045 Drosophila Genomics Resource Center DGRC#LD43045FlybaseID: FBcl0176185 pU6-Bbsl-chiRNA Addgene Addgene #45946 pHD-DsRed Addgene Addgene #51434 pUC19 Addgene Addgene #50005 pGeEX_6P1 Amersham AmershamCat# 27-4597-01 Software and algorithms Fiji Schindelin et al. 201370 http://fiji.sc Icy de Chaumont et al. 201271 http://icy.bioimageanalysis.org/ Ec-CLEM (Icy plugin) Paul-Gilloteaux et al. 201272 http://icy.bioimageanalysis.org/ plugin/ec-clem/ SerialEM Mastronade 200573 https://bio3d.colorado.edu/SerialEM/ SerialFIB Klumpe et al. 202174 https://github.com/sklumpe/SerialFIB gctf Zhang et al. 201675 N/A novaCTF Turo�nová et al. 201776 https://github.com/turonova/novaCTF novaSTA Turonova 202277 https://github.com/turonova/novaSTA GAPSTOPTM Turonova 202478 https://doi.org/10.5281/zenodo.10822455 Matlab The MathWorks, Inc. https://www.mathworks.com/ products/matlab.html Imod Kremer et al. 199679 https://bio3d.colorado.edu/imod/ AreTomo Zheng et al. 202280 https://msg.ucsf.edu/software MemBrain-Seg Lamm et al. 202281 https://github.com/teamtomo/ membrain-seg cryoCAT Turo�nová, B. https://github.com/ turonova/cryoCAT https://github.com/turonova/cryoCAT Relion 3.1 Zivanov et al. 201882 https://relion.readthedocs.io/ en/release-3.1/ ArtiaX Ermel et al. 202283 https://github.com/FrangakisLab/ArtiaX ChimeraX Meng et al. 202384 https://www.cgl.ucsf.edu/chimerax/ Python Python.org https://www.python.org/ SciPy Virtanen et al. 202085 https://scipy.org matplotlib Hunter et al. 200786 https://matplotlib.org/ napari Napari contributors 202598 https://napari.org/ Other Pelco easiGlow Ted Pella, Inc. N/A R1/4 SiO2 gold 200 mesh grids Quantifoil N/A Leica EM GP2 Leica Microsystems N/A Aquilos 1 cryo-FIB-SEM Thermo Scientific N/A Aquilos Leica CLEM Shuttle Thermo Scientific N/A Stellaris SP8 cryoCLEM Leica N/A (Continued on next page) ll OPEN ACCESS Cell 188, 5931–5946.e1–e9, October 16, 2025 e3 Article ..



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